Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines

Background: The World Health Organization views dengue fever as a significant worldwide public health threat in the tropics and subtropics. Dengue virus has four serotypes, DENG 1 to 4, which were responsible for outbreaks that occurred other parts of world from 1980s until now. Aims Objectives: Clinical manifestations molecular serological studies help determine inflammatory response events associated with severity. This study aimed define profiles some cytokines chemokines, well their serotyping. Materials Methods: Blood serum samples are collected, followed by serodiagnosis (NS1 IgM) through ELISA, serotyping detection real-time PCR SyBr green, an immunological tumor necrosis factor-alpha interferon-gamma (IFN-γ) on mother-hand chemokines CCL26 MCP1 ELISA. Statistical analysis was performed SPSS (version 22). Results: examined 70 seropositive samples, five NS1 positive 55 IgM positive. After accounting comorbidities patients’ demographic classifications, 21–30 age group maximum positivity 30%. mean concentration level IFN-γ is elevated at 1544.438 pg/mL. Conclusion: Dengue-positive patients have high ischemic heart disease rate, among diseases. An circulating osteopetrosis may cause cancer, tuberculosis, serious In aspects, elevation can be used predict severity infection.